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- 2025年07月16日
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- 技术资料
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999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70113665H
中文名称：Fisherbrand 标准锥形移液管
英文名称：PIPET MOHR 2X1/10ML 12/CS
货期：热销品现货，其他请咨询
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文献和实验Procedures for In Vitro Production of Bovine Embryos
all debris behind (repeat twice to assure that oocytes are clean of debris; Figure 10).Figure 10. An X-plate used to wash COCs, purchased from Fisher .Figure 11. Washing of COCs using an X-plate.Figure 12. Some instruments used to pick up embryos
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. The devittellinized embryos will sink to the bottom. Transfer the embryos to a vial with a wide bore pipet tip. Wash 3X with 90% MeOH/EGTA. Rehydrate embryos (0-2 and 1-3 hr embryos need not be rehydrated through paraformaldehyde, just leave
-well plate; Roughly 3x106 J2-3T3s (a fully confluent T75?) 1.5ml 10x reconstitution buffer 1.5ml 10x DMEM 12ml rat tail type 1 collagen (>3.8mg/ml) 10N NaOH Glacial acetic acid (in case) Method 1. Pre-chill pipettes, keep collagen
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