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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310448212
中文名称:
英文名称:Comb 200x0.35 mm 24 samples
货期:热销品现货,其他请咨询








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文献和实验Metabolic Labeling of Cells with 35S
1) Transfer to a 24 wells plate the desired colonies. 2) Once the cells are attached (at least 8 hours after tripsinizing them) add ~1 ml of DME (met-, cys-). 3) Add 3l of Met 35S, Cys 35S trans label. 4) Incubate at least 5 hours at 370C
100X dNTPs (25 mM each) 1 100 Yeast genomic DNA (0.2 ug/uL) 0.2 20 ddH2O 70.45 7045 AmpliTaq (5 U/uL) 0.35 35
lanes 2, 4,..., 24. 6. Load samples B1-B12 into alternating lanes 3, 5,..., 25. 7. Repeat this procedure for the remaining samples, such that two sequential rows of PCR reactions are loaded
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