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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70103-500-744
中文名称:
英文名称:MIDI SUB COMB 1.5 MM X 20 WELL
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文献和实验overnight (12-16 hours) at 37degC with shaking at 250 rpm. 4. Add 12.5 ml of sterile glycerol for a final concentration of 20%, and distribute the culture in 1.3 ml aliquots into 12 X 75 mm Falcon tubes. 5. Store glycerol cell stocks frozen
and further incubate overnight (12-16 hours) at 37℃ with shaking at 250 rpm. 4. Add 12.5 ml of sterile glycerol for a final concentration of 20%, and distribute the culture in 1.3 ml aliquots into 12 X 75 mm Falcon tubes. 5. Store glycerol cell stocks frozen at -70℃
Basic procedures for bacteria culture
microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20degC overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15 minutes at 4 degC, decant
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