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999
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北京泽平
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货号：T_70105586B
中文名称：试剂瓶
英文名称：BOTTLE 200ML
货期：热销品现货，其他请咨询
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文献和实验BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH
BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
of de-fined diatomaceous earth in guanidine-HCl (100 mg/ml) to each bottle. Allow the DNA to bind at room temperature for 5 minutes with occasional mixing. Centrifuge at 9,000 for 10 minutes in the RC5-B using the GS3 rotor. 10b. Decant the supernatant
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