赛默飞世尔Thermo Fisher X10 CRUCIBL
E MEDIUM FORM PORCELAIN 60ML (PACK OF 10) 货号：T_70312386497- ￥70 - 1400
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北京泽平
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货号：T_70312386497
中文名称：
英文名称：X10 CRUCIBLE MEDIUM FORM PORCELAIN 60ML (PACK OF 10)
货期：热销品现货，其他请咨询
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
A quick RNA mini-prep for Neurospora mycelial cultures
water bath, occasionally shaking, to get SDS into solution. This should take about 5 minutes. 6. Add 1.1 ml of 3M KCl, invert to mix, keep on ice for 10 min. Solution should form semi-solid, flocculent mass as K-SDS precipitate forms. 7. Spin
Cell counting with an hemacytometer.
dilution the easy count is: number of cells in two 1mm squares divided by 10 = cells x 106 / ml. (2 squares / 2 x 20 x10,000 / 1,000 = 106 cells / ml) (2 squares x 10 x 10,000 / 1,000 = 106 cells / ml) (2 squares x 100 = 106 cells / ml) (2 squares / 10
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