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- 2025年07月08日
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999
- 供应商：
北京泽平
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- 保修期：
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- 规格：
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货号：T_7017851-16
中文名称：
英文名称：SOD TARTRATE, 10% W/W, 500 ML
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文献和实验a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX
°C). Add 2.5 µl of 6X DNA loading buffer. Prepare the solution for the polyacrylamide gel. Mix 15 ml of 40% acrylamide/bisacrylamide stock solution, 12 ml of 5X TBE buffer, and 32.5 ml of H2O. Add 50 µl of TEMED and 500 µl of freshly prepared 10%
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
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