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999
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北京泽平
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热销品大量现货,其余请咨询
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1年
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EA
货号:T_70162-5PH
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英文名称:ORGANOCHLORINE PESTICIDES MIX
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文献和实验Large-scale yeast transformation
, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100
Small scale DNA preps for Neurospora crassa.
to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S-23) plus 500 microliters of isolation buffer (50 mM Tris-HCl pH 8, 170 mM EDTA pH 8, 1 N-lauroylsarcosine) to each tube and vortex
Small scale DNA preps for Neurospora crassa.
, and vortexing for an additional one minute. 4. Incubate the tubes at 65 degrees C for 5-7 min. 5. Add 300 microliters of 7.5 M ammonium acetate, invert each tube to mix the contents and incubate on ice for 10 min. 6. Spin for 3-5 min and pipette 700
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