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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
. autoclave, cool, and add: 1x MS vitamines (SIGMA #M-7150). Take 1ml of 1000x stock prepared by disolving 10.3gr in 100ml of water. antibiotic (kanamycin 50mg/l). Top agar: 1x Murashige&Skoog salts. 1% sucrose. adjust pH
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
by adding 500 U of thrombin to 18 mL of 0.9% saline and 2 mL of sterile deionized water, sterile filter through a 0.2 μm filter, aliquot into 500 μl and 250 μl aliquots and freeze at -80°C. 3) Prepare a 5% w/v Pluronics F-127 solution
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