赛默飞世尔Thermo Fisher 漏斗 FUNNEL 1

AFFINITY PURIFICATION OF ANTIBODIES

AFFINITY PURIFICATION OF ANTIBODIES (Fisher et al.,1988, Cell 54: 813-822) Conjugation of carrier (BSA) to peptide 1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate, pH 7.0. 2. Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

glycoconjugates utilizing on‐support oximation. Bioconjug. Chem. 15:890‐896.    Kawasaki, A.M., Casper, M.D., Prakash, T.P., Manalili, S., Sasmor, H., Manoharan, M., and Cook, P.D. 1999

TISSUE CULTURE ON COVERSLIPS

for the tissue to adhere to the surface; then add 1ml fresh media. The p-dish will be your back-up if the coverslips don't grow or get contaminated. D . Allow the tissue in the centrifuge tube to settle while you prepare and sterile filter the collagenase