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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
isothio-cyanate/cesium chloride gradients (Chirgwin et al. 1979 Biochem 18:5294-5299) and can be both expensive and time consuming. Often, however, needs arise where quantitatively smaller amounts of RNA are needed from many different samples, for example
A quick RNA mini-prep for Neurospora mycelial cultures
have been developed for the processing of large quantities of material. These typically involve multiple phenol extractions (Reinert et al. 1981 Mol. Cell Biol. 1:829-836) or guanadinium isothio-cyanate/cesium chloride gradients (Chirgwin et al. 1979 Biochem 18:5294
‐Diisopropylamino cyanoethyl phosphonamidic chloride (Cl‐P(OCE)Ni Pr 2 , ChemGenes) Saturated NaCl aqueous solution
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