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货号：T_70313435256
中文名称：
英文名称：X3 Labelling tape clear, write on, 25.4mm
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文献和实验Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
Screening lambda cDNA or genomic libraries
,and this will increase the efficiency of labelling considerably. - Boil the DNA to denature it for a few minutes.Briefly spin the tube and put on ice to cool.Add 6.6 µl 5X OLB,5 µl hot dATP (50 µCi),and 2 µl Klenow. - Allow the labelling reaction to proceed at room
/thawing here helps the following proteinase K digestions, and so is useful even if you don''t want to store the worms. 6. To the worms in 500µl TEN in a 1.5 ml eppendorf tube add: 25 µl 10% SDS (final conc. 0.5%) 2.5 µl 20 mg/ml Proteinase K
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