赛默飞世尔Thermo Fisher COMB 10 WEL

Agarose Gel Electrophoresis

in gel tray about 1 inch from one end of the tray and position the comb vertically such that the teeth are about 1-2 mm above the surface of the tray Pour 50EC gel solution into tray to a depth of about 5 mm. Allow gel to solidify about 20 minutes

Partial proteolysis with Staph. aureus V8 protease

mM Tris-HCl, pH 6.8, and 1mM EDTA. This is in Tilo's box 3, in the east Forma, #1. Generally, if you aren't sure how much enzyme to use, try 10, 50 and 100 nanograms of V8 protease. If V8 doesn't work for your protein, try papain

原创：western protocol

Tris(PH=6.8) 10% SDS 40 µl 10% ammonium persulfate 40 µl TEMED 7 µl C. Preparation of gel 1.  Assemble the glass plates and spacers (0.75 mm thick). 2.  Pour the running gel to about 1 cm below the wells of the comb (~3.2 ml). 3.  Seal with 1 ml