赛默飞世尔Thermo Fisher 漏斗 FUNNEL U

Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells

9. After the last centrifugation, discard the supernatant and resuspend PBMCs at 2 x 106 cells/mL in warm (37°C) R10 medium. Aliquot a maximum 107 (or 5 mL) cells per 50-mL PP conical tube and incubate overnight in a humidified atmosphere incubator preset to 37°C

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

Silica gel–purified S.4 a ( protocol 1 , step 12) Methanol (MeOH; Fisher) Ammonium fluoride (Aldrich

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

Imidazole, 99% pure (Sigma) tert ‐Butyldimethylsilyl chloride (TBDMS‐Cl), 98% pure (Acros) Hexanes, ACS grade (Fisher