赛默飞世尔Thermo Fisher GC AUTO SYR

单组分溶液配制

AcNa, pH 5.2   10mM 85.45 905 µl 4.53ml 9.05ml 18ml p~1.06g/ml Conc. Stock 1ml 5ml 10ml 20ml DTT 154.2g/M 2.2M 0.339g 1.696g 3.394g 6.787g AcNa, pH 5.2   10mM        

质谱的试剂用品、实验操作、仪器和样品选择方法

EP分装成5μl。 4. 50ml 100 mM NH4HCO3。 脱色酶切： 1. 切点将200μl tip头剪成吸口1.5mm左右的直径，MilliQ水涮洗一遍，吸100μl水后戳取斑点，将胶粒打入0.2ml EP管中，吸干水后再用MilliQ水洗涤两遍，吸干。 2. 脱色 180μl 50%MeOH/50mM NNH4HCO3脱色两次以上，每次半小时;180μl MilliQ水洗涤5分钟，180μ 50

Stripping Western Blots

) 11/01/2002 1. Coat inserts with ECM protein (e.g. Col I @ 10-20μg/ml, Fn @ 25μg/ml) by placing insert on a drop (~30μl) of diluted ECM solution, then adding an equal volume of the ECM solution to the inner chamber of the insert. Coat at RT