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- Thermo Fisher
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- T_701588513-32
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_701588513-32
中文名称：
英文名称：COND STD, 12.9 5S/CM, 1 L
货期：热销品现货，其他请咨询
;l_COND3kwZ:@=yj9m)A=Y(pf5+}h"E]cn^n!%oR4aEv%,<`"_9tGZe1!v%zkGV'riwr^K&`}1n1UW)0,;coC2KW+Y7Q:,BrS"+m*|XrWh>k+A4j.TcB
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文献和实验#______________ 1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________ X μl Nuclease free H2O to 10 μl 37℃ at least 1 hr After incubation, bring reaction volume up to 50 μl before cleaning up with spin column
MBP in kinase buffer. 12.) Wash the immunoprecipitate 3x with RIPA buffer and once with kinase buffer. 13.) Add 40 μl of the reaction mixture, along with 15 μCi of gamma-32P-ATP (3000Ci/mmol, NEN) to the washed beads and mix very gently
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
upon the amount of tissue used. 10. Count the cells using a hemacytometer and add the appropriate number to a nontreated polystyrene dish containing NEP complete medium. Cell density should be 1 x 106 cells per 6-cm plate, or 2 x 106 cells per 10-cm
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