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货号:T_701K953900-0010
中文名称:真空瓶
英文名称:BOTTLE VACUUM/PRSR 1000ML 1CS
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文献和实验the day of experiment.Dissolve in 100% EtOH) 1 mM Benzamidine 5 mM Iodoacetamide Whatman Paper Centrifuge Bottles Funnel Squeeze bottle Several Beakers Homogenizer (Fisher PowerGen 125) 50ml Corning tubes Ice Bucket & Ice Dounce
Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
【转贴】immunofluorescence staining
m l buffer for 24 well trays, 1 ml for 6 well trays, etc.) Remove formaldehyde fix, discard in toxic waste bottle, and wash X 3 with PEM buffer, about 2-5 min. per wash.Incubate 5-30 min. with PEM + 0.5% Triton X-100. This step permeablizes the cells
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