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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70122-045-582
中文名称:
英文名称:CULTURE TUBE PP BLUE 1000/CS
货期:热销品现货,其他请咨询








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文献和实验Transformation of Electrocompetent E. coli with Blue/White selection
to a bacteria tube. Repeat for additional DNA samples and control DNA. Rotate at 37ºC x30 min. to allow the bacteria to recover. Meanwhile plate LB/Amp plates with IPTG + X-gal if blue/white selection of colonies will be performed
).Don’t allow essential equipment to become out of calibration. Ensure microbiological safety cabinets are tested regularly.2.Protocol 1 - Aseptic Technique and Good Cell Culture PracticeAimTo ensure all cell culture procedures are performed
Basic procedures for bacteria culture
Protocol 1. Add the following reagents to a 0.5 ml microcentrifuge tube, in the order listed: sterile ddH2O q.s 10X kinase buffer 1 ul DNA x ul [[gamma]]-[32-P]-rATP 10 uCi T4 polynucleotide kinase 1 ul (3U/ul
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