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- Thermo Fisher
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- T_70311707529
- 2025年07月04日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311707529
中文名称：
英文名称：7,5M C-FLEX TUBG 4X5,6MM
货期：热销品现货，其他请咨询
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文献和实验PBS/FBS to each well, and mix by pipetting up and down. Immediately spin at 1500 RPM x2 min. and discard supernatant. Add 150 uL of 2º Ab (i.e. Streptavidin-TC) Master Mix. Incubate 30 min at 4ºC, then spin at 1500 RPM x5 min. Shake
is adjusted to 1.4M with 5M NaCl, 1/10 vol. of 10% CTAB added and samples incubated a further 10 min. at 65C. 4. Add 1 vol. chloroform:isoamyl alcohol, gently emulsify by inversion, incubate at 0C for 30 min. Spin 10 min. at 4C at rpm max. Transfer
Concentration of DNA by ethanol precipitation
for at least 10 minutes. It is possible to place the sample at -20°C overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15 min at 4°C, decant the supernatant, and drain inverted on a paper towel. 3. Add 80
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