赛默飞世尔Thermo Fisher X1000 Waste

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

1.5 ml    1.2 mM EDTA 0.5M stock 120 µl    1.1% Triton X-100 10% stock 5.5 ml    0.01% SDS 10% stock 50 µl

In situ hybridisation to alpha satellite sequences (chromosome specific)

than this you can put two slides face to face (acting as each other's coverslip) with 30m l of hyb solution.   50m l of Hybridisation solution Solution A 6mls de-ionised formamide 2mls 20 x SSC 2mls H2 O 2g dextran sulphate

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

(note 7).Materials & Reagents ChIP lysis buffer 30ml final volume:       1.5 ml 1M Hepes       1.05ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       24.15ml H20 ChIP high salt buffer       30ml final volume:       1.5ml 1M Hepes