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货号:T_70312537967
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英文名称:X100 Reservoir Axygen divided polypropylene, 25mL (pack of 100)
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文献和实验Large Scale Tubulin Preparation
KCl : Need ~10 liters for prewashing and eluting the PC column To make 1L of 10X CB : 151.2 grams PIPES, free acid 3.8 grams EGTA 2 ml of 1 M MgCl2 pH with KOH to pH 6.75, and bring up to 1 liter. Check pH at 1X is 6.7 Make
Large Scale Tubulin Preparation
should not be more than 25 mg/ml. Depolymerize on ice for 40' and then spin the depolymerization mix 30' at 40K in a 50.2Ti rotor at 4¡C. 10. Collect supernatant, measure concentration by Bradford and load onto the PC column (approx. 50 cm high X 5 cm ID = ~ 1L; Flow
). Pack at 45 ml/hour/cm cross-sectional area. For a 5 cm diameter column this is ~880 ml/hour or ~14.5 ml/min. After resin is packed, switch to pumping from the top. Run 7L of CB + 1M KCl through the column at 5-10 ml/min. 10. Wash with 10L CB
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