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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_7015886-58
中文名称：
英文名称：KCL COND STD, 50 US/CM, 250ML
货期：热销品现货，其他请咨询
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文献和实验Purification of recombinant sBRF M166L
. Elute with a 50 column volume gradient from 50 mM KCl to 550 mM KCl. Collect 0.5 ml fractions. BRF elutes in 2-3 fractions after about 1/3 of the gradient has completed at a conductivity of about 12 mS/cm-16mS/cm on the ATKA instrument. Expect 0.5- 1 mg
Purification of recombinant sBRF M166L
the column with 6 column volumes of B + 50 mM KCl. Elute with a 50 column volume gradient from 50 mM KCl to 550 mM KCl.Collect 0.5 ml fractions.BRF elutes in 2-3 fractions after about 1/3 of the gradient has completed at a conductivity of about 12 mS/cm-16
into several small screw-cap jars (~50 ml capacity) Store at room temperature 20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)
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