赛默飞世尔Thermo Fisher FRIO-TEMP H

Polyclonal and Monoclonal Antibodies

, boost again a month later). 3) 7 - 10 days later bleed the mouse and test serum using the assay which will be used for screening. If titre is not high enough, boost again two weeks after previous boost. Serum Preparation:

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

SDS-PAGE

and very gently squirt a 0.1% SDS overlay over the acrylamide solution (keeps oxygen away so that the top of the resolving gel polymerizes well). If you squirt this on gently enough you won't disturb the acrylamide solution, and a sharp line of refractive index