赛默飞世尔Thermo Fisher GRAD CYLIND

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

Silica gel–purified S.4 a ( protocol 1 , step 12) Methanol (MeOH; Fisher) Ammonium fluoride (Aldrich

Stripping Western Blots

-well plate; Roughly 3x106 J2-3T3s (a fully confluent T75?) 1.5ml 10x reconstitution buffer 1.5ml 10x DMEM 12ml rat tail type 1 collagen (>3.8mg/ml) 10N NaOH Glacial acetic acid (in case) Method 1. Pre-chill pipettes, keep collagen

Assays for DNA Damage

) 10 µg/ml RNase A in alkaline lysis solution (see recipe for the alkaline lysis solution; omit Triton X‐100 and adjust pH to 7) 1 mg/ml proteinase K in alkaline lysis