赛默飞世尔Thermo Fisher EDTA, 0.100

DNA Solutions （DNA分离）

5X Taq "T" termination mix: 250 uM dATP, 250 uM dCTP, 375 uM c7dGTP, 62.5 uM dTTP and 1.25 mM ddTTP in 5 mM Tris-HCl, pH 7.6 and 0.1 mM EDTA. 160 μl 20 mM dATP 160 μl 20 mM dCTP 480 μl 10 mM 7deaza-dGTP 40 μl 20 mM dTTP 3200 μl 5 mM ddTTP

小RNA的Northern BLOT

. Note: 300mA is the max on our power supply. 5’end-labeling of Probe:  1 μl 10x PNK buffer (fresh from NEB)  30 pmol probe DNA oligo  1.66 μl [γ -32P] ATP, 150 mCi/ml (= 250 μCi, 42 pmol) Vial

张峰手稿：基因工程技术之CRISPR-Cas9

used for PCR in this protocol. 4. Cloning enzymes: FastDigest Bbs I, FastDigest Age I, FastAP(Thermo Scientifi c/Fermentas, Pittsburg, PA, USA), Plasmid-Safe exonuclease (Epicentre, Madison, WI, USA), T7 DNA ligase. 5. 10× T4 DNA ligase buffer