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货号：T_701FBMB250
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英文名称：BOTTLE STR MEDIA FB 250ML CS24
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文献和实验Procedures for In Vitro Production of Bovine Embryos
indefinetely.Stock 16: Pen/Strep (10 ml). Thaw 100 ml bottle of pen/strep and aliquot 10 ml into sterile 13 ml tubes and store at -20°C indefinetely.Stocks 17-23 (prepare only if you make your own TL media)Stock 17: NaCl . Dissolve 6.665 g
【转贴】immunofluorescence staining
m l buffer for 24 well trays, 1 ml for 6 well trays, etc.) Remove formaldehyde fix, discard in toxic waste bottle, and wash X 3 with PEM buffer, about 2-5 min. per wash.Incubate 5-30 min. with PEM + 0.5% Triton X-100. This step permeablizes the cells
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Tert-amyl alcohol 5.0 ml (Fisher: A-730-1). Dissolve tribromethanol in Tert-amyl alcohol, then add to 200 ml distilled water. Place on a magnetic stirrer until solution is in one phase. Store in brown bottle and keep refrigerated until use
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