赛默飞世尔Thermo Fisher X6 VOLUMETR

Karyotyping of X. tropicalis

for 20 minutes. 5. Pipette the dorsal halves, carrying as little water with them as possible, into an Eppendorf tube containing 0.2 ml of 60% acetic acid in water. 6. Allow to stand for 5 minutes. 7. Pipette all of the tissue, carrying

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

  (1)  Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2)  Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

    Dissecting microscope;       Acid Tyrode's (e.g. Sigma: T1788);       Sterile plastic Petri dishes (100 x 15 mm);       M2 and KSOM medium in 1 ml syringes;       9'' Pasteur pipettes;       Mouth or Finger controlled pipette. Method: Place