赛默飞世尔Thermo Fisher AL23 TUBE (

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge

Procedures for In Vitro Production of Bovine Embryos

amino acids.3. Sterile filter medium through a 0.22 mm syringe filter into a sterile 10 ml beaker. Use immediately.CR1aa (an alternative culture medium) Note: the patent for this medium is held by Infigen1. Make CR1 stock (prepare in a 100 ml

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

of 20 mM HEPES Buffer in 0.9% saline solution. In a separate conical tube, create a T solution by adding 17 μl of the thrombin stock, and 1.3 μl of 2 N Ca solution to 135 μl of DMEM. See Table 1.    2)      In a third conical tube, prepare