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货号：T_7014620-16
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Phycoerythrin conjugation protocol
/ml (=0.7 ml) in PBS Add 16 µl SPDP (1.3 mg/ml in MeOH) Incubate at room temperature for 2.5 hours *** You must time steps 3 and 4 to finish at the same time, since the reaction products are unstable ***
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