赛默飞世尔Thermo Fisher National 样品

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Polyclonal and Monoclonal Antibodies

are immunized essentially as described above. Injections: 1. First in Freund's complete 100-200ug/1ml injection, two sites (over each shoulder). 2. Second 1 month later in Freund's INCOMPLETE as above. 3. Bleed 2 weeks later. Boost 2 weeks