赛默飞世尔Thermo Fisher CHEM PADS A

A quick RNA mini-prep for Neurospora mycelial cultures

. A 50 ml culture typically yields enough RNA for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel

A quick RNA mini-prep for Neurospora mycelial cultures

in aluminum foil and freeze in dry ice. Do not freeze in EtOH/dry ice bath because alcohol might seep through foil. Pads can be stored at -70°C for at least three weeks. 3. Wash a mortar and pestle thoroughly with warm water and Alconox (Fisher Scientific

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

is evaporated and the O 6 ?(benzotriazol?1?yl) ether is treated with Cs2 CO3 and an appropriate alcohol, at room temperature. On the other hand, for reaction with phenols, Cs2 CO3 and the appropriate phenol are added to the reaction mixture without evaporation