赛默飞世尔Thermo Fisher X100 VIALS,

Loss of Heterozygosity

microdissected cells in 20 µl proteinase K buffer.Incubate overnight at 37°C.B: Prepare the Glass PlatesTIP: Use Accuwipes for cleaning purposes, as they will not leave lint behind and are non-abrasive.Clean glass plates twice with glass cleaner.Repeat using 95

原创：western protocol

of a 10% gel 4 ml H2O 3.3ml 30% acrylamide/bisacrylamide (29:1 mix) 2.5 ml1.5M Tris(PH 8.8) 10% SDS 100 µl 10% ammonium persulfate 100 µl TEMED 8 µl 2.  Stacking gel: 4 ml 2.7ml water 1 ml 0.67ml 30% acrylamide/bisacrylamide (29:1 mix) 0.ml 1.0M

Northern Blotting方法(Howell Lab)

Make a 10 ng/µl dilution of the probe. Add 10 µl (100 ng) of probe to a 0.5 ml screw-cap eppendorf tube. Adjust volume to 28 µl with water. (ie, 7 µl of probe needs 21 µl H2 O.) Boil for 5 min and then chill on ice for 5 min.