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- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
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EA
货号:T_70310663062
中文名称:
英文名称:X4 BOTTLE,MASON POLYPROPYLENE2000ML (2115-2000) (PACK OF 4)
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Microarray Hybridization Protocol
block in the hot room). Quick chill on ice. In tube B combine: 80m l 5X 1st strand buffer 40 m l 0.1M DTT 10m l RNAse inhibitor 40U/m l (Boehringer 799025) 20m l 0.5 mg/ml Oligo(dT)12
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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