赛默飞世尔Thermo Fisher AMMONIUM VA

Replication timing by density transfer

. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration