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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
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EA
货号:T_701S-3845
中文名称:
英文名称:XYLENES (MIXED) 1.2ML
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文献和实验Immuno-Laser Capture Microdissection
by dehydration. The immunostained sections are ready for LCM. 1. Materials 1X phosphate-buffered saline, pH 7.4 Acetone 70%, 95%, 100% ethanol Xylenes, mixed, ACS grade (Sigma) DAKO Quick
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. Remove the chuck when the O.C.T. starts to turn white but before it's completely frozen. Place a second, smaller, drop of O.C.T. on top of the unfrozen portion of the first. Quickly place the embryos in the center of the drop
-well plate; Roughly 3x106 J2-3T3s (a fully confluent T75?) 1.5ml 10x reconstitution buffer 1.5ml 10x DMEM 12ml rat tail type 1 collagen (>3.8mg/ml) 10N NaOH Glacial acetic acid (in case) Method 1. Pre-chill pipettes, keep collagen
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