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英文名称:X2 BEAKERS TPX, 3000 ML Form (VE=2Stck.)
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文献和实验Arabidopsis transformation by floral dipping(拟南芥转化)
First prepare everything for the transformation. -Make inoculation medium: 5% sucrose (=50 g/l), 0.025% Triton X-100 (=250 µl per litre) in H2O (or in 1/2 strength ATS medium). You need about 300 ml for each Agro culture.
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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