赛默飞世尔Thermo Fisher PROBE TYPE

Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I

. When we use these specific oligonucleotides in a PCR, we generate a 366 bp amplification product from the wild-type DNA (T-target) and a 473 bp amplification product from the competitor DNA (Ccompetitor) (Fig.  1 b ). The PCR reactions were set up in 50 µl

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

.    Mayer, T. and Maier, M.E. 2007. Design and synthesis of a tag‐free chemical probe for photoaffinity labeling. Eur. J. Org. Chem. 2007:4711‐4720.   

Preparation of Tonsil Lysate

in a beaker that is kept on ice. Using the dounce homogenizer,homogenize the collected sample using an up & down and left & right hand motion.Do this at least 10 times to get a very fine sample. Note: To avoid spilling you sample,don’t use