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文献和实验Single and Double FISH protocols for Drosophila
in confocal microscopy., ed. Stephen Paddock, Humana Press Inc., in press. 1. MATERIALS 1.1 RNA Probe Preparation 1. 1.5 ml microcentrifuge tubes, autoclaved. 2. RNAse free DEPC treated or double-distilled water. 3. 5X T7/T
Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
Using Fluorescence Detection Basic Protocol 8: GC/MS Determination and Quantification of 15‐F2T Isoprostane Alternate Protocol 2: Immunoassay of F2‐Isoprostanes Basic Protocol
antibody. The amount will depend on the antibody and the abundance (total and relative) of the antigen, but a good starting point is 0.5μg antibody / 500μg lysate. 8) Incubate for 1-2hr at 4OC with rocking. Avoid overnight incubationsa. 9) Add 30-40μl
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