赛默飞世尔Thermo Fisher FAST RELEAS

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder

MACROPHAGE EXTRACTION: INTRAPERITONEAL LAVAGE

over the cell pellet. This must be done relative quickly and firmly so as no suspend cells properly, but not too forcefully so that you do not lyse the cells. Also, do not release fluid all the way down so that you don’t get bubbles. Be careful not to aspirate

High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.

% organic mix. Add Osmocote slow release fertilizer and maintain in a greenhouse at 22-28°C for two weeks. Continue to fertilize plants once a week with Miracle-Gro.    3)      For high-throughput screens, plant seeds directly in trays in sandy soil