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文献和实验【转贴】immunofluorescence staining
off the parafilm. Sealing the edge of the dish with tape or parafilm during incubation, plus placing a small wad of wetted kim-wipe in the dish, will keep the coverslips from drying out. Note: This protocol has been optimized for HeLa cells, and works
Procedures for In Vitro Production of Bovine Embryos
International (RPI)1-800-323-9814Pap pens (195505)Roche1-800-262-1640TUNEL kit (1684795)Sarstedt 1-800-257-51015 ml tubes (55-526-006) 13 ml tubes (62-515-006)Cell culture flasksRed cap T175 (83.1812.002)Red cap T75 (83.1813.302)Yellow cap T75 (83.1813.302
.1 RNA Probe Preparation (see Note 1 ) 1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates. 2. RNAse free water. 3. T7, T3 or SP6 RNA Polymerase (Fermentas Life Sciences, Burlington, ON, Canada
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