赛默飞世尔Thermo Fisher MIXED INDCT

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

labeled 3:2. Add 120ml of PTw to that aliquot. - 60ml in one bottle labeled 1:4. Add 240ml of Ptw to that aliquot.]- Hybridization buffer for oligonucleotide probes: 100 µg/ml autoclaved salmon sperm DNA, 50 µg/ml heparin, 0.1% Tween 20

常用缓冲液配制－2

to 1 liter store at 4oC Valap* Put 50 g Vaseline, 50 g lanolin, and 50 g paraffin (all available from Fisher) in a 1 L Pyrex beaker Heat on "low" setting on a hotplate, stirring occasionally, until all components are melted and well mixed Pour

Immuno-Laser Capture Microdissection

(A) and immunostained for CD45RO (B). Immunostaining highlights T cells within the germinal center and in the perifollicular area and makes architectural features more evident. Magnification x100. Figs. C & D : Microdissection from germinal centers