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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312070563
中文名称:
英文名称:FLASK,KJELDAHL,LONG NECK, PYREX GLASS 500ML SOCKET 24/29(FK500/3L)
货期:热销品现货,其他请咨询




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文献和实验Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
media. 6 3. Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. 4. Open the top of the suspension culture and use a sterile 1.0 ml transfer pipette to remove a 1.0 ml
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
at room temperature as follows: 2 x 2 min PBTw 3 x 20 min PBTw / BSA 2 x 5 min in Staining Buffer (see below). 24) Transfer gonads to staining buffer containing 4.5 ul NBT/ml, 3.5 ul X-phosphate/ml and 100 ng/ml DAPI and protect from light
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