赛默飞世尔Thermo Fisher AL23 INSLAT

A quick RNA mini-prep for Neurospora mycelial cultures

is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora

v468 chapter 5 糖原合成酶激酶-3 的抑制

for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin

Procedures for In Vitro Production of Bovine Embryos

amino acids.3. Sterile filter medium through a 0.22 mm syringe filter into a sterile 10 ml beaker. Use immediately.CR1aa (an alternative culture medium) Note: the patent for this medium is held by Infigen1. Make CR1 stock (prepare in a 100 ml