赛默飞世尔Thermo Fisher SAND SEA 50

真菌基因组DNA的提取方法

(ca. 100mg) and 500uL of extraction buffer. If using microfuge tubes, simply add the sand and extraction buffer: o    100mM Tris, pH8.0 o    10mM EDTA o    2% SDS o    100ug/mL Proteinase K o    1% B-mercaptoethanol  (tubes can be stored

Small scale DNA preps for Neurospora crassa.

to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S-23) plus 500 microliters of isolation buffer (50 mM Tris-HCl pH 8, 170 mM EDTA pH 8, 1 N-lauroylsarcosine) to each tube and vortex

Small scale DNA preps for Neurospora crassa.

, or until the cultures are saturated. 2. Spin the culture tubes for five minutes to compress the mycelia, then invert and blot the tubes on a paper towel to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S