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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
of gel, add 667 μL of F solution into a clean 50 mL centrifuge tube, followed by 167 μL of cell solution, and lastly add 167 μl of T solution. Pipette to mix solution together being careful not to introduce bubbles. Once solutions are mixed
Procedures for In Vitro Production of Bovine Embryos
all debris behind (repeat twice to assure that oocytes are clean of debris; Figure 10).Figure 10. An X-plate used to wash COCs, purchased from Fisher .Figure 11. Washing of COCs using an X-plate.Figure 12. Some instruments used to pick up embryos
mice (e.g., The Jackson Laboratory) 0.9% (w/v) NaCl 20 mg/ml Avertin working solution (see recipe
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