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北京泽平
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货号:T_70312433768
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英文名称:Snap-it seal,blk pre-slit tfe/sil septa, 100/Cs MA: C4011-55BLK
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文献和实验with the buffer. Add 1x TBE buffer in both reservoirs. Rinse out the wells with 1x TBE buffer, using a syringe. Pre run the gel at constant 65 mA until the gel reaches 50℃, about 1 hr. (Glue a temperature strip on the metal plate to monitor
in each well is suctioned out with a mouth pipette.Each base-specific sequencing reaction terminated with the short termination mix is loaded using a mouth pipette onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA
onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA. The reactions terminated with the long termination mix typically are divided in half and loaded onto two 0.15 mm X 70 cm X 20 cm denaturing
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