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英文名称:75 ML SEALING COMPOUND TUBE 75ML SILICONE RUBBER
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文献和实验Staining protocols for placental explant cultures
it to a 0.5 ml Eppendorf tube. Add blocking solution (PBS/4% BSA) and proceed as in Protocol 3. Incubations can be carried out on a roller mixer. After staining, transfer the gel fragment to a microscope slide and remove excess PBS by blotting with a tissue
Staining protocols for placental explant cultures
. Trim the gel using a scalpel retaining the central area with explant. Gently tease the remaining gel off the well surface using a small spatula. Use a pastette to transfer it to a 0.5 ml Eppendorf tube. Add blocking solution
【转贴】immunofluorescence staining
off the parafilm. Sealing the edge of the dish with tape or parafilm during incubation, plus placing a small wad of wetted kim-wipe in the dish, will keep the coverslips from drying out. Note: This protocol has been optimized for HeLa cells, and works
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