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- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312269309
中文名称:
英文名称:X2 POP UP RACK TUBE 15/50 ML PINK and 12 x 50mL tubes pink 255mm x 137mm x 72mm
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
5M stock 1.5 ml Fill up to a total volume of 50 ml with H2 O (8) Homogenize tissues on ice (10-12 strokes). Transfer the homogenate into a microfuge tube on ice.
).Transfer serum/white blood cells to 50 ml tube, underlay 12 ml RT Histopaque 1077.Spin 2500 rpm/30min at RT. To take mononuclear cells:Take off serum above band. (See attached figure, in preparation)Take mononuclear band + some Ficoll into two 15ml tubes
Transformation of E. coli by Electroporation
absolute ethanol. Ice 15 min. 3. Centrifuge at >12,000 x g for 15 min at 4° C. Carefully decant the supernatant. 4. Wash the pellet with 1 ml of 70% ethanol. Centrifuge at >12,000 x g for 15min at room temperature. Remove the supernate. 5. Air
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