赛默飞世尔Thermo Fisher COPLIN STAI

免疫组化石蜡包埋组织抗原修复

for 5 minutes in 100% ethanol Two times for 5 minutes in 95% ethanol Once for 5 minutes in 80% ethanol Approximately, 150 ml of the solutions are enough to cover the removable rack for 10 to 20 slides, using a 105 x 70 x 85 mm coplin jar. Solutions

Immunostaining of Paraffin Sections

), and then to PBS (2 x 5 min).  Insert sections in Coplin jars containing ‘TUF’ (Target Unmasking Fluid; Signet Labs [Dedham MA] #1050) preheated to 80-90°C.  Incubate in TUF for 10 min at 80-90°C.  Let jar cool 5 - 10 min, then rinse two times in ddH

Immunohistochemisty-Enzymatic Protocol

water, to make the Retrieval Solution. Heat the Retrieval Solution to 92 - 95° C. This may be accomplished by placing a polypropylene Coplin staining jar filled with Retrieval Solution into a water bath. Note: heating may crack glass staining