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货号：T_70114-955-257
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英文名称：PLASTIC JOINT CLAMP SZ34 10/PK
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文献和实验Culturing Chick Embryonic Myoblasts/Myotubes and Fibroblasts
3.> 4. 3 x crystylized trypsin in saline G, thaw and keep on ice. 5. STE, in 34o C water bath. 6. 'Preplate' from myotube culture, Day 2 step 22. Procedure 1. Rinse preplate with 10 ml STE. 2. Apply 5 ml cold 3x
#______________ 1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________ X μl Nuclease free H2O to 10 μl 37℃ at least 1 hr After incubation, bring reaction volume up to 50 μl before cleaning up with spin column
. Take the precipitate using a sterile transfer pipet (IWAKI 7801-002) (Do not suck! Wind the precipitate around the pipet.) or by centrifuging Dissolve the precipitate in (2/5 x X) ml TE containing 1 M NaCl and RNase A (1 to 10 ug/ml). Incubate at 55oC for 1 hr
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