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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311522173
中文名称:
英文名称:Sieve stainless steel 2.00mm
货期:热销品现货,其他请咨询




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文献和实验syringe with ice-cold PBS. 3. Fit a blunted 21 gauge needle (nip the bevel off with pliers). 4. Squirt about 3ml of the ice-cold PBS down the shaft of each excised femur. 5. Catch the tissue fragments in a hemispherical 80-mesh stainless steel sieve
stainless steel sieve perched on top of a plastic tube. 6. Rub the tissue fragments through the sieve using the bottom of another plastic tube, and rinse through with the remaining ice-cold PBS. 7. Centrifuge the sample. Isolation of bone marrow
Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh
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